RNA Preparation and Purification
Zyagen Labs Dog Universal Reference Total RNA extracted from wide variety of male and female Beagle DOG tissues. Made at industrial scales to minimize lot-to-lot variation. Ideal for microarray, NB, PCR and many other applications. 0.1mg/pK at 1mg/ml
Zyagen Universal Reference Total RNA is composed of highly pure intact total RNA isolated from wide variety of male & female tissues. Total RNA is extracted using modified guanidine thiocyanate method, treated with DNase & packed at a concentration of 1mg/ml. Features: High quality intact RNA without genomic DNA contamination. Consistent control for standard data set comparisons. Extracted from large variety of male & female tissues for broad gene coverage. Made at industrial scales to minimize lot-to-lot variation Quality Control: The integrity of RNA sample as indicated by intact ribosomal RNA is verified by agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. Applications: Reference RNA is ideal for microarray analysis, Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, microRNA studies, & purification of mRNA for library construction.
New England Biolabs, Inc. G(5')ppp(5')A RNA Cap Structure Analog – 1 umol
The 5' terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level. For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation, and a decrease in the formation of the initiation complex of mRNAs for protein synthesis. Certain prokaryotic mRNAs containing a 5' terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system. Also a cap requirement has been observed for splicing eukaryotic substrate RNAs. A method using E. coli RNA Polymerase primed with m7G(5' )ppp(5' )G or m7G(5' )ppp(5' )A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas. Larger amounts of capped RNAs are produced by transcription systems using SP6 RNA polymerase primed with m7G(5' )ppp(5' )G.
Research Products International Corp Direct-zol RNA MiniPrep w/Zymo-Spin IIC Columns, Capped, 200 Preps
The Direct-zol RNA MiniPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary.
Revvity Health Sciences Inc RNA Pico Sensitivity Assay Reagent Kit
RNA Pico Sensitivity Assay Reagent Kit
New England Biolabs, Inc. 3'-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog – 5 umol
Blocking of the 3' -hydroxyl of m7G with 3' -0-Me assures that the capped transcripts are homogeneous. The 3' - hydroxyl of the non-methylated G is the only 3' - hydroxyl available for initiation. The 5' terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level. For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation, and a decrease in the formation of the initiation complex of mRNAs for protein synthesis. Certain prokaryotic mRNAs containing a 5 terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system. Also a cap requirement has been observed for splicing eukaryotic substrate RNAs. A method using E. coli RNA Polymerase primed with m7G(5' )ppp(5' )G or m7G(5' )ppp(5' )A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas.
Bioworld RNase-Off Kit, 250 mL
RNase Off KitFor use in the removal of RNase A Contains: 250ml RNase Off Solution RNase Off Spray Bottle
Zymo Research Corporation Quick-RNA™ MidiPrep Kit (25 Preps)
The Quick-RNA Midiprep Kit is designed for the easy, reliable, and rapid isolation of up to 1 mg total RNA from cultured cells or tissue samples. The procedure combines a unique, single-step RNA extraction/binding buffer with Clean-Spin™column technology to yield high quality RNA in about 10 minutes. The Quick-RNA™ Midiprep Kit allows for the efficient recovery of total RNA from 103 to 108 cells or tissue. The method is easy: simply add the provided ZR RNA Buffer to extract total RNA from the cells of interest then purify the RNA using the provided Zymo-Spin™ V-E Columns. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.
Research Products International Corp Direct-zol RNA MicroPrep w/Zymo-Spin IC Columns, Capped, 200 Preps
The Direct-zol RNA MicroPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis.
LAMDA BIOTECH INC Column-Pure RNA Mini-Preps
A spin column-based system designed for total RNA isolation from animal tissues and cultured cells. The kit employs selective RNA binding to silica membrane technology for nucleic acid purification.